3h Thymidine
Mostrando 1-12 de 1327 artigos, teses e dissertações.
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1. Proliferation and osteogenic activity of fibroblasts induced with fibronectin
The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-
Braz J Med Biol Res. Publicado em: 17/08/2017
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2. Maintenance of the corneal epithelium is carried out by germinative cells of its basal stratum and not by presumed stem cells of the limbus
The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment we
Braz J Med Biol Res. Publicado em: 2014-06
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3. Vias de sinalização intracelular envolvidas no efeito proliferativo de relaxina em células de sertoli / Intracelular signaling pathways involved in the proliferative effect of relaxin in rat Sertoli cells
Relaxin is an insulin-related peptide that activates the G-protein coupled receptor RXFP1. Although relaxin plays an important role in female reproduction its physiological role in the male reproductive system is still unclear. We have previously demonstrated that relaxin and RXFP1 are expressed in testis (FILONZI et al., 2007; CARDOSO et al., 2010). Both re
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2011
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4. Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-10
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5. Efeito do armazenamento em água sobre a citotoxicidade de materiais resilientes para base de próteses. / Efect of water storage on the cytotoxicity of resilient liners
O objetivo deste estudo foi avaliar, por meio do teste quantitativo de incorporação de 3H-timidina, a citotoxicidade de materiais reembasadores resilientes em função do tempo de armazenamento em água e em função de um tratamento térmico. Doze corpos-de-prova de cada material reembasador resiliente (Dentusoft, Dentuflex, Trusoft e Ufi Gel P) e da resi
Publicado em: 2010
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6. Perfil de atividade da proteína tirosina fosfatase de baixa massa molecular relativa e da fosfatase ácida resistente ao tartarato em osteoblastos humanos durante o ciclo e diferenciação celular / Low molecular weight protein tyrosine phosphatase and tartrate resistant acide phosphatase activity in human osteoblasts during cell cycle and differentiation
Low molecular weight protein tyrosine phosphatase (LMW-PTP) and tartrate resistant acid phosphatase (TRAP) activity were determined in hFOB 1.19 human osteoblasts cell line during cell proliferation and differentiation. LMW-PTP and TRAP enzymatic activity were determined at 6, 18, 24, 36, 48 and 72 hours after fetal calf serum stimulation of subconfluent cul
Publicado em: 2005
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7. Comparison of the efficacy of biodegradable and non-biodegradable scintillation liquids on the counting of tritium- and [14C]-labeled compounds
The widespread use of ³H and 14C in research has generated a large volume of waste mixed with scintillation liquid, requiring an effective control and appropriate storage of liquid radioactive waste. In the present study, we compared the efficacy of three commercially available scintillation liquids, Optiphase HiSafe 3, Ultima-Gold™ AB (biodegradable) and
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-12
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8. Insulin impairs the maturation of chondrocytes in vitro
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular m
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-09
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9. Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently in
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-02
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10. Analise quantitativa, duração do ciclo do epitelio seminifero e produção espermatica do testiculo do gerbilo da Mongolia (Meriones unguiculatus)
The gerbil (Meriones unguiculatus) is a rodent native of the arid regions of Mongolia and China, is of special interest in biomedical research and has been bred in laboratory since 1960. Studies on the structure and function of the gerbil testis are sporadic and, moreover those on spermatogenesis are rare. This is the first study to perform a more detailed i
Publicado em: 2003
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11. Screening and fractionation of plant extracts with antiproliferative activity on human peripheral blood mononuclear cells
Three hundred and thirteen extracts from 136 Brazilian plant species belonging to 36 families were tested for their suppressive activity on phytohemaglutinin (PHA) stimulated proliferation of human peripheral blood mononuclear cells (PBMC). The proliferation was evaluated by the amount of [³H]-thymidine incorporated by the cells. Twenty extracts inhibited o
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-12
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12. Production and characterization of monoclonal antibodies to Brazilian isolates of bovine viral diarrhea virus
Three Brazilian isolates of bovine viral diarrhea virus (BVDV), antigenically distinct from the standard North American isolates, were selected to immunize BALB/c mice in order to obtain hybridoma cells secreting anti-BVDV monoclonal antibodies (mAbs). Two hybridoma clones secreting mAbs, reacting specifically with BVDV-infected cells (mAbs 3.1C4 and 6.F11),
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-12