Acidic Vacuoles
Mostrando 1-12 de 50 artigos, teses e dissertações.
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1. Ferulate Anion Intercalated into Zn/Al Layered Double Hydroxide: A Promising Intercalation Compound for Inhibition of Leishmania (L.) amazonensis
Ferulic acid (FERH) is known to exert leishmanicidal action against Leishmania (Leishmania) amazonensis. In this study, the ferulate anion (FER–) was intercalated into a layered double hydroxide (LDH) for FER– delivery during an antileishmania in vitro test. The LDH-FER– intercalation compound was prepared by coprecipitation and was characterized by X-
J. Braz. Chem. Soc.. Publicado em: 23/05/2019
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2. Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells
Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a “vacuolating factor” in H. influenzae supernatants w
Mem. Inst. Oswaldo Cruz. Publicado em: 10/10/2013
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3. Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549 / Adhesion, invasion and intracellular persistence of group B Streptococci in respiratory epithelial cells A549
Estreptococos do grupo B (EGB) comumente colonizam adultos saudáveis, sem sintomas, mas sob certas circunstâncias possui a capacidade de invadir tecidos do hospedeiro, evadir da detecção imunológica e causar doenças invasivas graves. Por conseguinte, os EGB continuam sendo uma das principais causas de mortalidade neonatal, pneumonia, sepse e meningite.
Publicado em: 2010
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4. Intracellular vacuoles in experimental acute pancreatitis in rats and mice are an acidified compartment.
The appearance of vacuoles inside acinar cells characterizes an early stage of development in different models of acute pancreatitis and, possibly, also in human disease. The vacuoles have been shown to contain both digestive and lysosomal enzymes. This abnormal admixture may have important implications for the pathogenesis of pancreatitis because the lysoso
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5. Parasitophorous vacuoles of Leishmania amazonensis-infected macrophages maintain an acidic pH.
Leishmania amastigotes are intracellular protozoan parasites of mononuclear phagocytes which reside within parasitophorous vacuoles of phagolysosomal origin. The pH of these compartments was studied with the aim of elucidating strategies used by these microorganisms to evade the microbicidal mechanisms of their host cells. For this purpose, rat bone marrow-d
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6. Barley aleurone cells contain two types of vacuoles. Characterization Of lytic organelles by use of fluorescent probes
Light microscopy was used to study the structure and function of vacuoles in living protoplasts of barley (Hordeum vulgare cv Himalaya) aleurone. Light microscopy showed that aleurone protoplasts contain two distinct types of vacuole: the protein storage vacuole and a lysosome-like organelle, which we have called the secondary vacuole. Fluorescence microscop
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7. Distribution of endosomal, lysosomal, and major histocompatability complex markers in a monocytic cell line infected with Chlamydia psittaci.
The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. Early Chlamydia-containing vacuoles expressed m
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8. Survival of Mycobacterium avium and Mycobacterium tuberculosis in Acidified Vacuoles of Murine Macrophages
Despite the antimicrobial mechanisms of vertebrate phagocytes, mycobacteria can survive within the phagosomes of these cells. These organisms use various strategies to evade destruction, including inhibition of acidification of the phagosome and inhibition of phagosome-lysosome fusion. In contrast to mycobacteria, Coxiella burnetii, the etiologic agent of Q
American Society for Microbiology.
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9. Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages.
We characterized the Mycobacterium marinum phagosome by using a variety of endocytic markers to follow the path of the bacteria through a mouse macrophage cell line. Using a laser confocal microscope, we found that the majority of viable M. marinum cells were in nonacidic vacuoles that did not colocalize with the vacuolar proton ATPase (V-ATPase), the calciu
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10. Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts.
Several biochemical and localization studies have shown that the acidic isoforms of the tobacco pathogenesis-related (PR) proteins, PR-1a, -1b and -1c are secreted to the extracellular spaces of leaves in response to pathogen infection or chemical treatment. Here we report the differential accumulation of these proteins within the vacuoles of specialized cel
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11. Regeneration of a Lytic Central Vacuole and of Neutral Peripheral Vacuoles Can Be Visualized by Green Fluorescent Proteins Targeted to Either Type of Vacuoles1
Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic ly
American Society of Plant Physiologists.
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12. Purification, Properties, and Molecular Cloning of a Novel Ca2+-Binding Protein in Radish Vacuoles1
To understand the roles of plant vacuoles, we have purified and characterized a major soluble protein from vacuoles of radish (Raphanus sativus cv Tokinashi-daikon) taproots. The results showed that it is a novel radish vacuole Ca2+-binding protein (RVCaB). RVCaB was released from the vacuolar membrane fraction by sonication, and purified by ion exchange and
American Society of Plant Physiologists.