Adenoviral Vectors
Mostrando 1-12 de 115 artigos, teses e dissertações.
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1. DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
Abstract Objective: To determine the role of the dishevelled binding antagonist of beta catenin 1 (DACT1) in the cytoskeletal arrangement of cardiomyocytes in atrial fibrillation (AF). Methods: The DACT1 expression and its associations with the degree of fibrosis and β-catenin in valvular disease patients were analyzed by immunohistochemistry and Masson�
Braz. J. Cardiovasc. Surg.. Publicado em: 23/09/2019
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2. Synergism/complementarity of recombinant adenoviral vectors and other vaccination platforms during induction of protective immunity against malaria
The lack of immunogenicity of most malaria antigens and the complex immune responses required for achieving protective immunity against this infectious disease have traditionally hampered the development of an efficient human malaria vaccine. The current boom in development of recombinant viral vectors and their use in prime-boost protocols that result in en
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-08
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3. Construção de ferramentas para estudo da possível interação entre interferon-beta e p53. / Construction of tools for study of the possible interaction between interferon-beta and P53.
Formation of tumors it must to combinations of factors. The p53 pathway has an essential role in proliferation control and apoptosis. The interferon-beta (IFNb) is important in modulation of the immunologic response, in the antitumoral effect and in the apoptotic impact in tumor cells. According to literature, IFNb activate the p53 transcription and componen
Publicado em: 2009
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4. Use of adenoviral vectors in the identification of genetic complementation group of patients with Xeroderma pigmentosum um and animals deficient in DNA repair. / Uso de vetores adenovirais na identificação de grupo de complementação gênica de pacientes com Xeroderma pigmentosum e em animais deficientes em reparo de DNA.
One of the most versatile mechanisms of DNA repair is the nucleotide excision repair (NER). Genetic defects in NER can generate different syndromes. Among these, Xeroderma pigmentosum) presents the highest sensitivity to sunlight, resulting in a large increase in the incidence of skin cancer, especially in areas exposed to the sunlight, and in some cases, pr
Publicado em: 2008
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5. Development and evaluation of immunization tools based on the adenovirus fiber knob modified with the C4 domain of HIV gp120 glycoprotein. / Desenvolvimento e avaliação de ferramentas de imunização baseadas na região globular da fibra adenoviral modificada com o domínio C4 da glicoproteína gp120 do HIV.
HIV glycoprotein gp120 has conserved domains, one of them being the C4 domain. This region is involved in the recognition of the CD4 marker in target cells and antibodies that recognize this domain can block HIV infection. Previously, the C4 domain was introduced in the adenovirus fiber knob. As the adenovirus fiber stimulates de immune system, we decided to
Publicado em: 2008
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6. XPA DNA repair gene modulation in human cell lines by genetic vectors / Modulação da expressão do gene de reparo de DNA xpa por meio de vetores genéticos em células humanas
A integridade do DNA é ameaçada pelos efeitos lesivos de inúmeros agentes físicos e químicos que podem vir a comprometer sua função. Um dos mais versáteis e estudados mecanismos de reparo de DNA é o reparo por excisão de nucleotídeos (NER). Este mecanismo remove lesões que causam distorções na dupla fita de DNA, incluindo dímeros de pirimidina
Publicado em: 2001
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7. Efficient and Selective Gene Transfer into Primary Human Brain Tumors by Using Single-Chain Antibody-Targeted Adenoviral Vectors with Native Tropism Abolished
The application of adenoviral vectors in cancer gene therapy is hampered by low receptor expression on tumor cells and high receptor expression on normal epithelial cells. Targeting adenoviral vectors toward tumor cells may improve cancer gene therapy procedures by providing augmented tumor transduction and decreased toxicity to normal tissues. Targeting req
American Society for Microbiology.
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8. Adenoviral vectors can impair adrenocortical steroidogenesis: Clinical implications for natural infections and gene therapy
Recombinant adenoviral vectors are effective in transferring foreign genes to a variety of cells and tissue types, both in vitro and in vivo. However, during the gene transfer, they may alter the principal function and local environment of transfected cells. Increasing evidence exists for a selective adrenotropism of adenovirus during infections and gene tra
The National Academy of Sciences.
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9. Successful expression of human factor IX following repeat administration of adenoviral vector in mice.
Adenoviral vectors can direct high-level expression of a transgene, but, due to a host immune response to adenoviral antigens, expression is of limited duration, and repetitive administration has generally been unsuccessful. Exposure to foreign proteins beginning in the neonatal period may alter or ablate the immune response. We injected adult and neonatal (
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10. An improved helper-dependent adenoviral vector allows persistent gene expression after intramuscular delivery and overcomes preexisting immunity to adenovirus
Helper-dependent adenoviral vectors deleted of all viral coding sequences have shown an excellent gene expression profile in a variety of animal models, as well as a reduced toxicity after systemic delivery. What is still unclear is whether long-term expression and therapeutic dosages of these vectors can be obtained also in the presence of a preexistin
The National Academy of Sciences.
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11. Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex.
We describe an efficient cloning system utilizing adenoviral DNA-protein complexes which allows the directional cloning of genes into adenoviral expression vectors in a single step. DNA-protein complexes derived from a recombinant adenovirus (AVC2.null) were isolated by sequential use of CsCl step gradients followed by isopycnic centrifugation in a mixture o
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12. Administration of helper-dependent adenoviral vectors and sequential delivery of different vector serotype for long-term liver-directed gene transfer in baboons
The efficiency of first-generation adenoviral vectors as gene delivery tools is often limited by the short duration of transgene expression, which can be related to immune responses and to toxic effects of viral proteins. In addition, readministration is usually ineffective unless the animals are immunocompromised or a different adenovirus serotype is used.
The National Academy of Sciences.