Agarose And Chitosan
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
Berbamine loaded chitosan-agarose microspheres were prepared using a water-in-oil emulsion technique. Optimum preparing parameters were determined by orthogonal experiments as follows: ratio of berbamine to chitosan (w/w) is 1:10; percentage of emulsifier (span 80, v/v) is 6%; volume of glutaraldehyde is 2 mL; and reaction temperature is 70 ºC. Under these
Polímeros. Publicado em: 09/11/2012
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2. Imobilização da enzima β-galactosidase de Kluyveromyces fragilis em agarose e quitosana utilizando diferentes protocolos de ativação
The objective of this work was stabilize and immobilize β-galactosidase from activated agarose and chitosan supports. Initially, it was evaluated the buffer type, ionic strength and bivalent ions Mn2+ and Mg2+ on the hydrolytic activity of the enzyme using as substrates lactose and o-NPG (o-nitrophenyl galactopyranoside). Then the enzyme was covalently
Publicado em: 2009
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3. Imobilização e estabilização de D-Hidantoinase para a produção de N-Carbamoil-D-Fenilglicina
Immobilization and stabilization of enzymes increases their potential for use in industrial scale. D-hydantoinases (dihidropirimidina amidrohidrolase EC 3.5.2.2) catalyze the hydrolysis of D-hydantoins, generating the corresponding Ncarbamoil- D-amino acid and are used in the production of D-amino acids, including Dphenylglycine and D-p-hydroxyphenylglycine.
Publicado em: 2008
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4. Concentração e purificação de Beta-amilase de extrato de soja por adsorção em gel de afinidade quitosana-fenilboronato
B-amylase (E.C 3.2.1.2) is an enzyme that hydrolyses non-reduced ends of starch and maltooligossacarides producing units of maltose. The main industrial application of this enzyme is the production of high maltose syrup. The traditional sources of B-amylase are plants and microorganism. Since its main use is found in food processing, the production cost of t
Publicado em: 1999
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5. Chitosan lactate as a nonviral gene delivery vector in COS-1 cells
The purpose of this research was to evaluate chitosan lactate (CL) of different molecular weights (MWs) as a DNA complexing agent for its efficiency in transfecting COS-1 cells (green monkey fibroblasts) and its effect on cell viability compared with polyethylenimine (PEI), a commercially available cationic polymer. CL and chitosan base dissolved in dilute a
Springer-Verlag.