Amplified Clinical
Mostrando 1-12 de 786 artigos, teses e dissertações.
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1. Antibacterial Effects of Cuminum cyminum Extract Against Enterococcus Faecalis Biofilms From Clinical Isolates
Abstract Objective: To compare the antibacterial efficacy of Cuminum cyminum (cumin) extract and 2% chlorhexidine. Material and Methods: E. faecalis was isolated from non-vital teeth with chronic apical abscess. Samples were then bred in the ChromAgar medium. Subsequently, E. faecalis bacteria’s DNA extraction was performed. DNA was then amplified by con
Pesqui. Bras. Odontopediatria Clín. Integr.. Publicado em: 13/01/2020
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2. Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples
ABSTRACT At present, there is no standardized marker that is routinely used in clinical laboratories to diagnose coccidioidomycosis. Thus, the goals of this study were to obtain a sequence characterized amplified region (SCAR) marker for the identification of Coccidioides spp., evaluate its specificity and sensitivity in fungal DNA-spiked blood and sputum sa
Braz J Infect Dis. Publicado em: 25/11/2019
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3. Problemas éticos na saúde bucal no contexto da Atenção Primária à Saúde
Resumo Diante da ausência de estudos voltados às questões éticas vivenciadas por profissionais de Saúde Bucal no contexto da Atenção Primária à Saúde (APS) e como primeira etapa de adaptação com validação do Inventário de Problemas Éticos na Atenção Primária à Saúde (IPE-APS) para a saúde bucal, objetiva-se, com esta pesquisa, desvelar
Physis. Publicado em: 16/09/2019
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4. Usefulness of a multiplex PCR for the rapid identification of Candida glabrata species complex in Mexican clinical isolates
ABSTRACT Candida glabrata complex includes three species identified through molecular biology methods: C. glabrata sensu stricto , C. nivariensis and C. bracarensis . In Mexico, the phenotypic methods are still used in the diagnosis; therefore, the presence of C. nivariensis and C. bracarensis among clinical isolates is still unknown. The aim of this study w
Rev. Inst. Med. trop. S. Paulo. Publicado em: 08/08/2019
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5. Genotypes of clinical varicella-zoster virus isolates from Manaus, Brazil
Abstract INTRODUCTION: Vaccination against varicella-zoster virus (VZV) has been effective and safe in countries that routinely administer the vaccine. Brazil began universal VZV vaccination in 2013. This study aimed to identify VZV genotypes present in Manaus, Brazil prior to widespread immunization. METHODS: Vesicular lesions or cerebral-spinal-fluid sam
Rev. Soc. Bras. Med. Trop.. Publicado em: 16/05/2019
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6. Genotyping of Leptospira interrogans isolates from Mexican patients
ABSTRACT The aim of this study was genotypically characterize Leptospira sp. clinical isolates from Mexico which were previously identified as Leptospira interrogans serovar Pomona (POM) by phenotypic methods. The Random Amplified Polymorphic DNA (RAPD) method was used for DNA amplification with five oligonucleotides. A dendrogram was constructed using the U
Rev. Inst. Med. trop. S. Paulo. Publicado em: 06/05/2019
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7. Diagnosis of canine leptospirosis: evaluation of two PCR assays in comparison with the microagglutination test
RESUMO: O diagnóstico definitivo da leptospirose canina é geralmente realizado demonstrando a seroconversão em amostras do paciente no período agudo e de convalescença por serologia. No entanto, a aplicação de técnicas de PCR pode contribuir para a confirmação de casos suspeitos num período de tempo mais curto. O objetivo deste estudo foi avaliar
Pesq. Vet. Bras.. Publicado em: 02/05/2019
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8. Association between col1a2 Polymorphism and the Occurrence of Pelvic Organ Prolapse in Brazilian Women
Resumo Objetivo Avaliar o polimorfismo rs42524 do gene pró-colágeno tipo I alfa (α) 2 (COL1A2) como fator relacionado ao desenvolvimento de prolapso de órgãos pélvicos (POP) em mulheres brasileiras. Métodos O estudo envolveu 112 mulheres com POP nos estádios III e IV (grupo caso) e 180 mulheres com POP nos estádios zero e I (grupo controle). Out
Rev. Bras. Ginecol. Obstet.. Publicado em: 15/04/2019
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9. Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR
RESUMO: O objetivo deste estudo foi genotipar isolados clínicos de Pythium insidiosum da América do Sul utilizando polimorfismos de nucleotídeo único (SNP) de sequências de rDNA. Anteriormente, um multiplex-PCR baseado em SNP foi capaz de distinguir P. insidiosum em três diferentes clados. Dessa forma, utilizamos este método para avaliar isolados cl�
Cienc. Rural. Publicado em: 17/01/2019
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10. Histophilus somni-associated syndromes in sheep from Southern Brazil
Abstract Histophilus somni is a Gram-negative bacterium that is associated with a disease complex (termed histophilosis) that can produce several clinical syndromes predominantly in cattle, but also in sheep. Histophilosis is well described in North America, Canada, and in some European countries. In Brazil, histophilosis has been described in cattle with re
Braz. J. Microbiol.. Publicado em: 2018-09
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11. Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer
ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of br
Braz. arch. biol. technol.. Publicado em: 25/06/2018
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12. Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods that facilitate a rapid and accurate diagnosis is cru
Mem. Inst. Oswaldo Cruz. Publicado em: 26/02/2018