Antisense Transcripts
Mostrando 1-12 de 219 artigos, teses e dissertações.
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1. Analysis of energetically biased transcripts of viruses and transposable elements
RNA interference (RNAi) is a natural endogenous process by which double-stranded RNA molecules trigger potent and specific gene silencing in eukaryotic cells and is characterized by target RNA cleavage. In mammals, small interfering RNAs (siRNAs) are the trigger molecules of choice and constitute a new class of RNA-based antiviral agents. In an efficient RNA
Genet. Mol. Biol.. Publicado em: 13/11/2012
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2. Efeitos do pareamento no perfil de expressão gênica do parasita Schistosoma mansoni / Effects of pairing on the gene expression profiles of the parasite Schistosoma mansoni
Schistosomiasis is a chronic and debilitating disease. Schistosoma represents the only class of trematodes with a dioecious life. A continuous pairing with the male is essential for female sexual maturation. Adult females from uni-sexual infections are underdeveloped, have body atrophy and an immature reproductive system. To study the mechanisms involved in
Publicado em: 2010
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3. Uso de técnicas computacionais no estudo da transcrição e regulação gênica em Homo sapiens e Mus musculus / Use of computational methods to study the transcription and gene regulation in Homo sapiens and Mus musculus
Genes, nucleotide sequences necessary for the synthesis of functional molecules, are transcribed and regulated by extremely complex cellular and molecular processes. To understand when and in which tissues the genes are expressed, their functional isoforms, control regions and the factors involved in gene regulation is one of major challenges of modern molec
Publicado em: 2008
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4. Identificação e análise de expressão de RNAs intrônicos não codificadores humanos / Identification and expression analysis of human intronic noncoding RNAs
In this work, we show large-scale studies of antisense noncoding RNAs transcribed from intronic regions of human genes. The correlation of expression levels of some intronic transcripts to the degree of tumor differentiation in prostate cancer points to the biological relevance of these messages in complex diseases such as cancer. We also evaluated the exist
Publicado em: 2007
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5. Construção de cassetes de expressão para silenciamento gênico de fatores antinutricionais da soja, via interferência por RNA / Construction of expression cassettes for RNAi-based silencing of genes encoding antinutritional factors in soybean seeds
Soybean is one of the most important crops in the world extensively used as a food and feed source. However, the proteins present in soybean seeds are not considered ideal because they contain low amounts of the essential amino acids methionine and lysine. Adverse nutritional and other effects following consumption of raw soybean meal have been attributed to
Publicado em: 2006
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6. Deletion Derivatives of the MuDR Regulatory Transposon of Maize Encode Antisense Transcripts but Are Not Dominant-Negative Regulators of Mutator Activities
The maize MuDR/Mu transposable elements are highly aggressive, and their activities are held in check by host developmental and epigenetic mechanisms. The Mutator regulatory element, MuDR, produces both sense and antisense transcripts. We have investigated the impact of the presence of antisense transcripts on the abundance of the corresponding sense message
American Society of Plant Biologists.
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7. Antisense Transcripts With FANTOM2 Clone Set and Their Implications for Gene Regulation
We have used the FANTOM2 mouse cDNA set (60,770 clones), public mRNA data, and mouse genome sequence data to identify 2481 pairs of sense–antisense transcripts and 899 further pairs of nonantisense bidirectional transcription based upon genomic mapping. The analysis greatly expands the number of known examples of sense–antisense transcript and nonant
Cold Spring Harbor Laboratory Press.
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8. The abundance of sterile transcripts in Giardia lamblia
The protozoan parasite Giardia lamblia synthesizes a diverse and surprisingly abundant array of sterile transcripts unable to code for proteins. Random sampling of cDNAs from two evolutionarily divergent Giardia strains indicates that ∼20% of cDNAs in the libraries represent polyadenylated sterile transcripts. RNase protection analysis and northern blot hy
Oxford University Press.
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9. Naturally occurring antisense: Transcriptional leakage or real overlap?
Naturally occurring antisense transcription is associated with the regulation of gene expression through a variety of biological mechanisms. Several recent genome-wide studies reported the identification of potential antisense transcripts for thousands of mammalian genes, many of them resulting from alternatively polyadenylated transcripts or heterogeneous t
Cold Spring Harbor Laboratory Press.
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10. Antisense transcripts with rice full-length cDNAs
In this study, 687 sense-antisense transcript pairs from 32,127 full-length rice cDNA sequences were identified by aligning the cDNA sequences with rice genome sequences. The large number of pairs suggests that gene regulation by antisense transcripts occurs in plants and not only in animals.
BioMed Central.
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11. Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.
We describe a general antisense strategy to inhibit target gene expression. The substitution of a cis-acting ribozyme for a polyadenylylation signal in an antisense expression vector results in the nuclear retention of RNAs and the efficient degradation of their targets. We demonstrate the utility of this system in polyoma virus, where early-strand RNA level
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12. Differential expression of a Clostridium acetobutylicum antisense RNA: implications for regulation of glutamine synthetase.
The Clostridium acetobutylicum glutamine synthetase (GS) DNA region is characterized by a downstream promoter, P3, oriented toward the glnA gene, which controls the transcription of an RNA complementary to the start of the glnA mRNA. Expression of the predicted 43-base antisense RNA was demonstrated in C. acetobutylicum and Escherichia coli cells containing