Aprotinin
Mostrando 1-12 de 87 artigos, teses e dissertações.
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1. Key Points for Curbing Cardiopulmonary Bypass Inflammation
PURPOSE: Cardiopulmonary bypass (CPB) procedures are thought to activate systemic inflammatory reaction syndrome (SIRS). Strategies to curb systemic inflammation have been previously described. However, none of them is adequate, since "curbing" the extent of the inflammatory response requires a multimodal approach. The aim of the present mini-review is to
Acta Cir. Bras.. Publicado em: 2016
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2. Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues
The aim of this work was to study the production of fibrinolytic protease by Bacillus subtilis I-2 on agricultural residues. Molasses substantially enhanced (63%) protease production (652.32 U/mL) than control (398.64 U/mL). Soybean meal supported maximum protease production (797.28 U/mL), followed by malt extract (770.1 U/mL), cotton cake (761.04 U/mL), gel
Braz. arch. biol. technol.. Publicado em: 2014-10
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3. Purification and functional characterization of a new non-hemorrhagic metalloprotease from Bothrops moojeni snake venom / Purificação e caracterização bioquímica da moozincina, uma metaloprotease dependente de zinco presente na peçonha da serpente Bothrops moojeni (Caiçaca)
Bothrops snake venoms contain proteases that contribute to the local effects seen after envenoming. In this study, a non-hemorrhagic metalloprotease was isolated from the venom of B. moojeni. The enzyme was isolated by a combination of ion exchange and gel filtration chromatographies and named Moozincin. The enzyme was purified to homogeneity as judged by it
Publicado em: 2010
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4. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae
Members of the high temperature requirement A (HtrA) family of chaperone proteases have been shown to play a role in bacterial pathogenesis. In a recent report, we demonstrated that the gene ML0176, which codes for a predicted HtrA-like protease, a gene conserved in other species of mycobacteria, is transcribed by Mycobacterium leprae in human leprosy lesion
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-12
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5. Partial purification and characterization of serine and cysteine proteases of Sitophilus zeamais (Coleoptera: Curculionidae) / Purificação e caracterização parciais de serino e cisteíno proteases de Sitophilus zeamais (Coleoptera: Curculionidae)
Serine and cysteine proteases of a susceptible and two resistant strain (one with a cost associated with resistance and other without cost) of the maize weevil Sitophilus zeamais were partially purified using an affinity column of aprotinin-agarose . The highest purification factor was obtained for the resistant no-cost strain, 51.20x, with a yield of 14.8%
Publicado em: 2009
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6. Cromatografia negativa em Sepharose-TREN como tecnica de purificação de proteinas adicionadas artificialmente a extrato de soja / Negative chromatography on Sepharose-TREN as a technique of proteins purification artificially added to soybean extract
In recent years, plants have been studied as bioreactors for the production of recombinant proteins with industrial and pharmaceutical interest. When compared to other expression systems, plants have several advantages, such as the possibility of posttranlationals modification, low production cost, low risk of contamination by human pathogens, and the accumu
Publicado em: 2009
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7. Purification and characterization of the aprotinin from porcine lung. / Purificação e caracterização da aprotinina obtida de pulmão suíno.
Aprotinin, an acid stable serine proteinase inhibitor with a molecular mass of 7 kDa, is used as a reagent or drug. The purification of the aprotinin from porcine lungs was the main objective of this work. Three procedures were used. The first one utilized the trypsin-agarose column. The tangential ultra filtration and trypsin-Sepharose column were used in t
Publicado em: 2008
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8. Protective effects of the antioxidant Ginkgo biloba extract and the protease inhibitor aprotinin against Leiurus quinquestriatus venom-induced tissue damage in rats
Oxidative stress and proteases have been implicated in several diseases and extensive evidence indicates that antioxidants and protease inhibitors help prevent organ functional damage. Leiurus quinquestriatus (LQQ) scorpion venom causes cellular injuries that may lead to multiple organ failure. Thus, the capability of the antioxidant "natural standardized ex
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2006-04
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9. Efeitos da aprotinina em crianças com cardiopatia congênita acianogênica operadas com circulação extracorpórea / Effects of aprotinin in children with acianogenic congenital heart disease submitted to correction with extracorporeal circulation
Introduction. Aprotinin seems to reduce the need for transfusion, the inflammatory process and myocardial damage after extracorporeal circulation (ECC). Material and Methods. A prospective randomized study was conducted on children aged 30 days to 4 years submitted to correction of acyanogenic congenital heart disease with ECC and divided into two groups: Co
Publicado em: 2006
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10. Pharmacological characterization of rat paw edema induced by Cerastes gasperettii (cerastes) venom
Inflammatory response induced by the venom of the Arabian sand viper Cerastes gasperettii was studied by measuring rat hind-paw edema. Cerastes gasperettii venom (CgV, 3.75-240 µg/paw), heated for 30s at 97°C, caused a marked dose and time-dependent edema in rat paw. Response was maximal 2h after venom administration and ceased within 24h. Heated CgV was r
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2006
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11. Purification of recombinant aprotinin produced in transgenic corn seed: separation from CTI utilizing ion-exchange chromatography
Protein expression in transgenic plants is considered one of the most promising approaches for producing pharmaceutical proteins. As has happened with other recombinant protein production schemes, the downstream processing (dsp) of these proteins produced in plants is key to the technical and economic success of large-scale applications. Since dsp of protein
Brazilian Journal of Chemical Engineering. Publicado em: 2005-09
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12. Purificação e caracterização cinética da calicreína tecidual do rato (rk1) com os inibidores da tripsina:: benzamidina, 4-aminobenzamidina e 4-nitrobenzamidina, e estudo comparativo com dados da literatira para a inibição da tripsina
The present study is to purify and determine the kinetic characteristics of the tissue calicreína mouse (rK1) to further develop selective enzyme inhibitors for human calicreínas. The principle is summed up and characterized the compound 4-nitrobenzamidina. Next, purified to rK1 by steps: DEAE-Sepharose (ion-exchange chromatography) and aprotinin-Sepharose
Publicado em: 2005