Aqueous Biphasic System
Mostrando 1-12 de 16 artigos, teses e dissertações.
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1. Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction
Abstract Aqueous two-phase systems (ATPS) are formed by mixtures of chemical species that, under certain conditions, separate into two immiscible phases, with water as the major component in global terms and, at lower concentrations, polymers, salts, ionic liquids and alcohols, depending on the system type. Different parameters influence ATPS equilibrium suc
Food Sci. Technol. Publicado em: 16/05/2019
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2. Diterpenes and a new benzaldehyde from the mangrove plant Rhizophora mangle
ABSTRACT This work describes the isolation, by high-speed counter-current chromatography, of the diterpenes manool, jhanol and steviol and the benzaldehyde p-oxy-2-ethylhexyl benzaldehyde from the stilt roots hexane extract of the mangrove plant Rhizophora mangle L., Rhizophoraceae. For this, a non-aqueous biphasic solvent system composed of hexane–acetoni
Rev. bras. farmacogn.. Publicado em: 2017-04
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3. Equilíbrio líquido-líquido de sistemas aquosos constituídos por copolímero tribloco e sal em diferentes temperaturas / Liquid-liquid equilibrium of aqueous systems consisting of triblock copolymer and salt at different temperatures
Phases diagrams of aqueous biphasic systems (ABS) formed by triblock copolymer+salt+H2O were construed at three temperatures: The ABS L64+Na2SO4+H2O and L64+Li2SO4+H2O at 5, 10 and 25 C and ABS L35+Na2CO3+H2O, F68+Na3C6H5O7+H2O, F68+Na2C4H4O6+H2O, F68+Na2CO3+H2O and F68+Na2C4H4O4+H2O at 10, 25 and 40 C. The effect of temperature on the position of the binoda
Publicado em: 2009
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4. Biorreciclagem de hexano e estudo de reações de óxido-redução usando plantas comestíveis / Biorecycling of hexane and study of oxido-reduction reactions using edible plants
The present work had as main goals the use of enzymatic reactions to degrade laboratory residues and to synthesize chiral alcohols. In the first part, it was carried out a screening of microorganisms and hydrolytic enzymes aiming the biorecycling of hexane from laboratory residues (a mixture of hexane-ethyl acetate). This misture is widely employed to purify
Publicado em: 2008
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5. Extração dos íons Cu(II), Cd(II), Fe(III) e Zn(II) utilizando um sistema aquoso bifásico constituído pelo copolímero L35 e o sal Li2SO4 / Extraction of the ions Cu(II), Cd(II), Fe(III) and Zn(II) using aqueous biphasic system constituted by the copolymer L35 and the salt Li2SO4
In this work, the extraction behavior of the metal ions Cd (II), Cu(II), Fe(III) and Zn(II) has been separately investigated in aqueous biphasic systems (ABS) formed by the triblock copolymer (Pluronic L35,
= 1900.g.mol-1) and the salt Li2SO4, in the presence of extractant agents either soluble or insoluble in water. The extractant thiocyanate (SCN-), io Publicado em: 2007
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6. Extração e recuperação da bromelina em sistemas de duas fases aquosas PEG4000-POLICAJU
Aqueous two-phase system explores the possibility of solutions of two polymers or one polymer and one salt separating into two immiscible aqueous phases, when certain critical concentrations are exceeded. This work was characterised by the KA W ASE recovery index analysis of the bromelain of pineapple juice, using a pulsed column for three sizes of tie lines
Publicado em: 2004
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7. Intensificated process of hydrolysis of penicillin G and purification of the products in a multi-stage couter-current reactor / Processo intensificado de hidrolise enzimatica de penicilina G e purificação dos produtos em reator multi-estagio e contra-corrente
This work studies the enzymatic hydrolysis of penicillin G (PenG) into 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA). In a multi-stage countercurrent reactor and at low pH, the enzymatic reaction takes place in the aqueous phase and the products are separated between the aqueous phase and organic phase (butyl acetate - BuAc). Furthermore, 6-AP
Publicado em: 2004
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8. Estudo do equilibrio liquido-liquido, da partição de insulina e da pre-purificação da proteina de fusão precursora da insulina humana em sistemas aquosos bifasicos do tipo PEG/Sal / Study of liquid-liquid equilibrium, the partition of insulin and pre-purification of the fusion protein precursor of human insulin in aqueous biphasic systems of the type PEG / salt
In this work the primary purification of a fusion protein precursor of human insulin and produced by recombinant E. coli has been studied using aqueous two-phase systems. The fusion protein was provided by the pharmaceutical company Biobrás S.A (Montes Claros/Brazil) and was dissolved in 8M area. Partitioning essays of fusion protein were investigated in th
Publicado em: 2003
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9. Extração de proteinas em um sistema de duas fases aquosas numa micro-coluna pulsada
Aqueous two-phase systems have found widespread use in biochemical research for separation and purification of macromolecules, cells and cell partic1es. In recent years the aqueous two-phase systems have also found applications in various areas of biotechnology, specially when utilising low-cost biphasic systems, as the new aqueous two-phase system based on
Publicado em: 2001
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10. Caracterização do sistema bifasico aquoso polietilenoglicol - goma do cajueiro
Aqueous two-phase systems have found widespread use in biochemical research for separation and purification of macromolecules, cells and cell particles. In recent years the aqueous two-phase systems have also found applications in various areas of biotechnology, specially when utilizing low-cost biphasic systems, as the new aqueous two-phase system based on
Publicado em: 1999
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11. Selection of Xenobiotic-Degrading Microorganisms in a Biphasic Aqueous-Organic System
Microbial selection on mixtures of chlorinated and nonchlorinated compounds that are poorly soluble in water and/or toxic to growing microbial cells was examined in both biphasic aqueous-organic and monophasic aqueous systems. A biphasic system in which silicone oil was used as the organic phase permitted the acceleration of acclimation, leading to rapid sel
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12. Purification of Junin virus by an aqueous biphasic polymer system.