3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

AUTOR(ES)

Vandenberghe, A

RESUMO

32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.

Documentos Relacionados

• 3'-Terminal sequence of human parainfluenza virus 3 genomic RNA.
• The 3'-terminal sequence of mitochondrial 13S ribosomal RNA.
• Sequence of the 3'-terminal 21 nucleotides of yeast 17S ribosomal RNA.
• Nucleotide sequence of the 3'-terminal region of artichoke mottled crinkle tombusvirus RNA.
• Probing sequence-specific RNA recognition by the bacteriophage MS2 coat protein.