300-MHz Nuclear Magnetic Resonance Study of Oxytocin in Aqueous Solution: Conformational Implications
AUTOR(ES)
Brewster, A. I. Richard
RESUMO
The 300-MHz nuclear magnetic resonance spectrum of the peptide hormone, oxytocin, in water, was obtained. Extensive nuclear magnetic resonance spindecoupling experiments, including those involving irradiation of the α-proton resonance under the water peak, studies using partially deuterated hormone derivatives, and nuclear magnetic resonance studies of some precursor peptides to oxytocin, permitted us to assign most of the proton resonance of the hormone unambiguously. The JNα values and temperature dependence of the chemical shift of the peptide amide and carboxamide protons of oxytocin were also obtained. These studies indicate that, in aqueous solution, oxytocin is a flexible molecule possessing several different conformations. Corresponding studies of [4-leucine]oxytocin were also made, with similar results and conclusions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=427333Documentos Relacionados
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