5′ Nuclease PCR Assay To Detect Yersinia pestis

AUTOR(ES)

Higgins, James A.

FONTE

American Society for Microbiology

RESUMO

The 5′ nuclease PCR assay uses a fluorescently labeled oligonucleotide probe (TaqMan) to rapidly detect and quantitate DNA templates in clinical samples. We developed a 5′ nuclease PCR assay targeting the plasminogen activator gene (pla) of Yersinia pestis. The assay is species specific, with a detection threshold of 2.1 × 105 copies of the pla target or 1.6 pg of total cell DNA. The assay detected Y. pestis in experimentally infected Xenopsylla cheopis fleas and in experimentally infected monkey blood and oropharyngeal swabs. The TaqMan assay is simple to perform and rapid and shows promise as a future field-adaptable technique.

Documentos Relacionados

• Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
• Automated 5′ Nuclease PCR Assay for Identification of Salmonella enterica
• Development of a Fluorogenic 5′ Nuclease PCR Assay for Detection of the ail Gene of Pathogenic Yersinia enterocolitica
• Detection of Viable Listeria monocytogenes with a 5′ Nuclease PCR Assay
• Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella