A 5′-Adenosine Monophosphate-Dependent Adenylate Cyclase and an Adenosine 3′:5′-Cyclic Monophosphate-Dependent Adenosine Triphosphate Pyrophosphohydrolase in Dictyostelium discoideum

AUTOR(ES)

Rossomando, Edward F.

RESUMO

Cell aggregation in Dictyostelium discoideum appears to involve the production and detection of 3′:5′-cyclic AMP. Two pertinent catalytic activities have been studied in concentrates from D. discoideum purified 50- to 100-fold. They are (i) adenylate cyclase and (ii) ATP pyrophosphohydrolase. ATP pyrophosphohydrolase activity converts ATP to 5′-AMP and pyrophosphate. The presence of 5′-AMP is an absolute requirement for adenylate cyclase activity. The presence of 3′:5′-cyclic AMP is an absolute requirement for ATP pyrophosphohydrolase activity. Both activations, particularly that of ATP pyrophosphohydrolase, show narrow ranges of specificity and display significant cooperativities.

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