A Biosensor Using Poly(4-Aminophenol)/acetylcholinesterase modified graphite electrode for the detection of dichlorvos

AUTOR(ES)

Melo, Edmar Isaías, Franco, Diego Leoni, Afonso, André Santiago, Rezende, Hélen Cristine, Brito-Madurro, Ana Graci, Madurro, João Marcos, Coelho, Nívia Maria Melo

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2011-12

RESUMO

The properties of poly(4-aminophenol) modified graphite electrode as material for the immobilization of acetylcholinesterase were investigated by the Cyclic Voltammetry, Electrochemical Impedance Spectroscopy and Atomic Force Microscopy. The polymer was deposited on graphite electrode surface by the oxidation of 4-aminophenol and then acetylcholinesterase was immobilized on the surface of the electrode. The biosensor coupled in the continuous flow system was employed for the detection of dichlorvos. The detection and quantification limits were 0.8 and 2.4 μmol L-1 dichlorvos, respectively. Graphite electrodes modified with the poly(4-aminophenol) showed to be an efficient and promising material for immobilization of acetylcholinesterase enzyme. The proposed method requires simple parts which are easy to build, involves only one biosensor and the potentiometric detection is simple.

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