A dicistronic construct allows easy detection of human CFTR expression from YAC DNA in human cells.
AUTOR(ES)
Vassaux, G
RESUMO
We have made a dicistronic construct where the picornaviral internal ribosome-entry site (IRES) driving the expression of the beta-geo gene has been inserted into the 3'untranslated region of the human CFTR gene present in a YAC. When introduced into the human cell line Caco-2 expressing the CFTR gene, the expression of the dicistronic gene can be detected by lacZ staining and follows the accumulation of the endogenous CFTR mRNA upon differentiation of the cells. These data demonstrate that this IRES-based approach presents an alternative to mRNA in situ hybridisation and allows detection of expression in an autologous system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147010Documentos Relacionados
- A DNA helicase from human cells.
- Analysis of a YAC with human telomeres and oriP from epstein-barr virus in yeast and 293 cells.
- Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.
- Inducible gene expression by DNA rearrangements in human cells.
- Regulation of CFTR expression and function during differentiation of intestinal epithelial cells.
