A fast and sensitive method for detecting specific viral RNA in mammalian cells.
AUTOR(ES)
Paeratakul, U
RESUMO
A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253120Documentos Relacionados
- The mechanism of viral carcinogenesis by DNA mammalian viruses: viral-specific RNA in polyribosomes of adenovirus tumor and transformed cells.
- A quick and efficient method for the recovery of plasmid or viral DNA from mammalian cells.
- A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells.
- Genetic recombination between mouse type C RNA viruses: a mechanism for endogenous viral gene amplification in mammalian cells.
- A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells.