A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues.

AUTOR(ES)

Powell, C T

RESUMO

By employing S1 nuclease mapping of total RNA isolated from porcine cerebral cortex, cerebellum, hypothalamus, pituitary, kidney, liver, pancreas, intestine, and antral mucosa, we have investigated gastrin gene expression in these tissues. Our results show that a gastrin gene is expressed only in the antral mucosal and pituitary tissues. Based on the amount of gastrin specific probe protected from S1 nuclease digestion in the presence of a given weight of total RNA, the amount of gastrin mRNA present in pituitary is approximately 330 times lower than in antral mucosa. These findings help establish the tissue distribution of gastrin gene expression.

Documentos Relacionados

• Endothelin-1 mRNA is widely expressed in porcine and human tissues.
• Chicken myosin IB mRNA is highly expressed in lymphoid tissues.
• Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase): cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues.
• Identification and analysis of the gene encoding human PC2, a prohormone convertase expressed in neuroendocrine tissues.
• A glucose transport protein expressed predominately in insulin-responsive tissues.