A geminivirus replication protein is a sequence-specific DNA binding protein.
AUTOR(ES)
Fontes, E P
RESUMO
The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by using an immunoprecipitation assay for AL1:DNA complex formation. In this assay, a monoclonal antibody against AL1 precipitated AL1:TGMV DNA complexes, whereas an unrelated antibody failed to precipitate the complexes. Competition assays with homologous and heterologous DNAs established the specificity of AL1:DNA binding. AL1 produced by transgenic tobacco plants and by baculovirus-infected insect cells exhibited similar DNA binding activity. The AL1 binding site maps to 52 bp on the left side of the common region, a 235-bp region that is highly conserved between the two TGMV genome components. The AL1:DNA binding site does not include the putative hairpin structure that is conserved in the common regions or the equivalent 5' intergenic regions of all geminiviruses. These studies demonstrate that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=160156Documentos Relacionados
- Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein.
- Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin.
- The human foamy virus Bel-1 transcription factor is a sequence-specific DNA binding protein.
- The enhancer factor R of Epstein-Barr virus (EBV) is a sequence-specific DNA binding protein.
- Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein.
