A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
AUTOR(ES)
van der Marel, G A
RESUMO
A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exemplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320613Documentos Relacionados
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