A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
AUTOR(ES)
Angelini, Leticia Mara Lima, Silva, Amanda Ribeiro Martins da, Rocco, Lucas de Freitas Coli, Milagre, Cintia Duarte de Freitas
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
31/03/2015
RESUMO
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
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