A highly conserved, 5' untranslated, inverted repeat sequence is ineffective in translational control of the alpha 1(I) collagen gene.

AUTOR(ES)

Bornstein, P

RESUMO

An inverted repeat sequence, extending from the 5' untranslated region of the first exon through the translation initiation codon, is highly conserved in the alpha 1(I), alpha 2(I) and alpha 1(III) collagen genes of mammals and birds. It has been suggested that this sequence functions in translational control of collagen gene expression. When the upstream axis of the dyad of symmetry was deleted, the efficiency of translation of transcripts from a human alpha 1(I) collagen-bovine growth hormone fusion gene was unchanged in either transiently or stably transfected cells. Furthermore, mRNA levels were not affected when the same deletion was transferred to a collagen-human growth hormone fusion gene in which the collagen sequence retained the first intron. Examination of human alpha 1(I) DNA, extending from the start of transcription to the start of translation, by the DNAse I protection procedure revealed evidence for protein binding to a sequence just upstream of the inverted repeat sequence but not to the inverted repeat itself. Our studies therefore indicate that this highly conserved DNA sequence does not function generally in translational or transcriptional control of type I procollagen synthesis.

Documentos Relacionados

• A highly conserved, 5′ untranslated, inverted repeat sequence is ineffective in translational control of the α1(I) collagen gene
• A highly conserved intronic sequence is involved in transcriptional regulation of the alpha 1(I) collagen gene.
• LAP (NF-IL6) transactivates the collagen alpha 1(I) gene from a 5' regulatory region.
• 5' untranslated sequences are required for the translational control of a yeast regulatory gene.
• Regulatory elements in the first intron contribute to transcriptional control of the human alpha 1(I) collagen gene.