A homolog of Escherichia coli RecA protein in plastids of higher plants.
AUTOR(ES)
Cerutti, H
RESUMO
Studies of chloroplast DNA variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. However, no studies have been done of the biochemical pathways involved. Using a part of a cyanobacterial recA gene as a probe in Southern blots, we have found homologous sequences in total DNA from Pisum sativum and Arabidopsis thaliana and in a cDNA library from Arabidopsis. A cDNA was cloned and sequenced, and its predicted amino acid sequence is 60.7% identical to that of the cyanobacterial RecA protein. This finding is consistent with our other results showing both DNA strand transfer activity and the existence of a protein of the predicted molecular mass crossreactive with antibodies to Escherichia coli RecA in the stroma of pea chloroplasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=49857Documentos Relacionados
- RecA protein stimulates homologous recombination in plants.
- Stable transformation of plastids in higher plants.
- A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes.
- Dual role for Escherichia coli RecA protein in SOS mutagenesis.
- New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.
