A large product of cell-free translation of messenger RNA coding for corticotropin.
AUTOR(ES)
Nakanishi, S
RESUMO
Polyadenylate-containing RNA prepared from the membrane fraction of bovine anterior pituitary gland was shown to direct the synthesis of a large translation product related to corticotropin (adrenocorticotropic hormone) in a cell-free system derived from wheat germ. This product was identified by immunoprecipitation with specific antibody against corticotropin, followed by electrophoretic analysis of the dissociated immunoprecipitate on sodium dodecyl sulfate-polyacrylamide gel. Further evidence for the identity of the translation product was provided by the presence of a common peptide in the chymotryptic digest of [35S]methionine-labeled cell-free product and in that of authentic corticotropin. The molecular weight of the translation product was estimated to be approximately 35,000, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results indicate that corticotropin messenger RNA directs the cell-free synthesis of a product that contains the amino acid sequence of corticotropin but is much larger than this hormone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431443Documentos Relacionados
- Cell-Free Translation of Paramyxovirus Messenger RNA
- Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
- Cell-Free Translation of Highly Purified Adenovirus Messenger RNA
- Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization
- Cell-Free Translation of Maternal Messenger RNA from Sea Urchin Eggs