A mutation, adjacent to gene amdS, defining the site of action of positive-control gene amdR in Aspergillus nidulans.
AUTOR(ES)
Hynes, M J
RESUMO
In Aspergillus nidulans the acetamidase enzyme is inducible by omega-amino acids, sources of acetyl-coenzyme A, and benzoate. The amdR (or intA) gene is a positive-control gene involved in omega-amino acid induction only. A cis-acting mutation amdI93 located in a complex controlling region adjacent to the acetamidase structural gene was found to abolish induction by omega-amino acids but not induction by other sources of induction. As predicted, this mutation was epistatic to constitutive amdR alleles but did not affect the expression of mutations in other regulatory genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=293985Documentos Relacionados
- The amdR product and a CCAAT-binding factor bind to adjacent, possibly overlapping DNA sequences in the promoter region of the Aspergillus nidulans amdS gene.
- Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.
- Identification of the sites of action for regulatory genes controlling the amdS gene of Aspergillus nidulans.
- Cloning and analysis of the positively acting regulatory gene amdR from Aspergillus nidulans.
- Sequence and functional analysis of the positively acting regulatory gene amdR from Aspergillus nidulans.