A new class of errant DNA polymerases provides candidates for somatic hypermutation.
AUTOR(ES)
Tippin, B
RESUMO
The mechanism of somatic hypermutation of the immunoglobulin genes remains a mystery after nearly 30 years of intensive research in the field. While many clues to the process have been discovered in terms of the genetic elements required in the immunoglobulin genes, the key enzymatic players that mediate the introduction of mutations into the variable region are unknown. The recent wave of newly discovered eukaryotic DNA polymerases have given a fresh supply of potential candidates and a renewed vigour in the search for the elusive mutator factor governing affinity maturation. In this paper, we discuss the relevant genetic and biochemical evidence known to date regarding both somatic hypermutation and the new DNA polymerases and address how the two fields can be brought together to identify the strongest candidates for further study. In particular we discuss evidence for the in vitro biochemical misincorporation properties of human Rad30B/Pol iota and how it compares to the in vivo somatic hypermutation spectra.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1087690Documentos Relacionados
- Transcription, beta-like DNA polymerases and hypermutation.
- The reverse transcriptase model of somatic hypermutation.
- Stepwise intraclonal maturation of antibody affinity through somatic hypermutation.
- In vivo and in vitro studies of immunoglobulin gene somatic hypermutation.
- Expression of error-prone polymerases in BL2 cells activated for Ig somatic hypermutation
