A new hydrogen-bonding potential for the design of protein–RNA interactions predicts specific contacts and discriminates decoys

AUTOR(ES)

Chen, Yu

FONTE

Oxford University Press

RESUMO

RNA-binding proteins play many essential roles in the regulation of gene expression in the cell. Despite the significant increase in the number of structures for RNA–protein complexes in the last few years, the molecular basis of specificity remains unclear even for the best-studied protein families. We have developed a distance and orientation-dependent hydrogen-bonding potential based on the statistical analysis of hydrogen-bonding geometries that are observed in high-resolution crystal structures of protein–DNA and protein–RNA complexes. We observe very strong geometrical preferences that reflect significant energetic constraints on the relative placement of hydrogen-bonding atom pairs at protein–nucleic acid interfaces. A scoring function based on the hydrogen-bonding potential discriminates native protein–RNA structures from incorrectly docked decoys with remarkable predictive power. By incorporating the new hydrogen-bonding potential into a physical model of protein–RNA interfaces with full atom representation, we were able to recover native amino acids at protein–RNA interfaces.

Documentos Relacionados

• Early hydrogen-bonding events in the folding reaction of ubiquitin.
• Protein–RNA interactions: a structural analysis
• Hydrogen-bonding effects and 13C-NMR of the DNA double helix.
• A comparison of two phage coat protein-RNA interactions.
• Visualization of Protein-RNA Interactions in Cytoplasmic Polyhedrosis Virus