A new method for the isolation of recombinant baculovirus.
AUTOR(ES)
Patel, G
RESUMO
An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectable marker. The viral DNA maintained its ability to replicate in insect cells. An efficient and rapid selection system was set up, to isolate viral recombinants in yeast; DNA from selected yeast colonies was transfected into insect cells to obtain recombinant virus. We demonstrate the utility of this system by isolating recombinant viruses that express two different members of the CREB/ATF family of transcription factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310331Documentos Relacionados
- An efficient procedure for the isolation of recombinant baculovirus.
- Expression of equine herpesvirus 1 glycoprotein D by using a recombinant baculovirus.
- Rabies diagnostic reagents prepared from a rabies N gene recombinant expressed in baculovirus.
- Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.
- Expression of two forms of carp gonadotropin alpha subunit in insect cells by recombinant baculovirus.
