A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.
AUTOR(ES)
Markiewicz, W T
RESUMO
A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146946Documentos Relacionados
- Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.
- A new and fast method for preparing high quality lambda DNA suitable for sequencing.
- New energy transfer dyes for DNA sequencing.
- Solid-phase synthesis of chelate-labelled oligonucleotides: application in triple-color ligase-mediated gene analysis.
- A rapid method for isolating high quality plasmid DNA suitable for DNA sequencing.
