A new PCR based method for the generation of nested deletions.
AUTOR(ES)
Whitcomb, J M
RESUMO
We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable for DNA sequencing. However, it should also be possible to use this technique for a number of other purposes: generating deletions for the analysis of eukaryotic promoters, extending cDNA clones in the 5' direction, cloning the insertion sites of retroviral proviruses and transposons, and analyzing intron/exon boundaries.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310022Documentos Relacionados
- A novel system for the rapid generation of precise DNA deletions.
- pYUM1118: a new plasmid vector for generating deletions.
- A simple method for preparing pools of synthetic oligonucleotides with random point deletions.
- New device and method for capture, reverse transcription and nested PCR in a single closed-tube.
- Rapid generation of nested chromosomal deletions on mouse chromosome 2