A Novel Approach to High Accuracy of Video-Based Microrheology
AUTOR(ES)
Wu, Pei-Hsun
FONTE
The Biophysical Society
RESUMO
Video-based particle tracking monitors the microscopic movement of labeled biomolecules and fluorescent probes within a complex cellular environment. Information gained from this technique enables us to extract the dynamic behavior of biomolecules and the local mechanical properties inside the cell from a tracked particle's mean-square displacement (MSD). However, MSD measurements are highly susceptible to static error introduced by noise in the image acquisition process that leads to an incorrect positioning of the particle. Static error can mask the subtle effects from the local microenvironment on the MSD and potentially generate misleading conclusions about the biophysical properties of cells. An approach that greatly increases the accuracy of MSD measurements is presented herein by combining experimental data with Monte Carlo simulations to eliminate the inherent static error. This practical method of static error correction greatly advances particle-tracking techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2712022Documentos Relacionados
- Video-based cognitive-behavioral intervention for COVID-19 anxiety: a randomized controlled trial
- A Low-Cost Video-Based Reflectometer for Selective Detection of Cu2+ Using Paper-Based Colorimetric Sensors
- Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging
- Chest pain score: a novel and practical approach to angina pectoris. A diagnostic accuracy study
- A novel approach based on multiple criteria decision aiding methods to cope with classification problems
