A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator

AUTOR(ES)

Rhee, Sangkee

FONTE

National Academy of Sciences

RESUMO

A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix–turn–helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 Å thereby bending the DNA by ≈35°. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.

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