A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging
AUTOR(ES)
Botchway, Stanley W.
FONTE
Portland Press Ltd.
RESUMO
A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1199672Documentos Relacionados
- Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice
- In vivo spin-label murine pharmacodynamics using low-frequency electron paramagnetic resonance imaging.
- Development of a method for screening short-lived proteins using green fluorescent protein
- A method for correlating in vivo prostate magnetic resonance imaging and histopathology using individualized magnetic resonance -based molds
- In vivo functional chronic imaging of a small animal model using optical-resolution photoacoustic microscopy
