A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication
AUTOR(ES)
Kubota, Yumiko
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=196047Documentos Relacionados
- RING-LIKE CORNEAL DEPOSITS IN A CASE OF CONGENITAL SPHEROCYTOSIS*
- Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels
- The initiator protein E1 binds to the bovine papillomavirus origin of replication as a trimeric ring-like structure.
- A Prokaryotic Condensin/Cohesin-Like Complex Can Actively Compact Chromosomes from a Single Position on the Nucleoid and Binds to DNA as a Ring-Like Structure†
- Calyculin A induces contractile ring-like apparatus formation and condensation of chromosomes in unfertilized sea urchin eggs.