A partial cDNA clone for human apolipoprotein B.
AUTOR(ES)
Deeb, S S
RESUMO
A human liver cDNA library was screened for sequences coding for apolipoprotein B (apo B), the major protein of human low density lipoproteins. A mixture of synthetic oligonucleotides (26 bases long) coding for an amino acid sequence known to exist in apo B was used as a hybridization probe. A clone was identified that had a cDNA insert of 593 base pairs and that contained sequences coding for a peptide of 24 residues that had earlier been isolated from apo B by limited proteolysis. The entire nucleotide sequence of the cDNA insert consists of one open reading frame coding for 197 amino acids. Apo B-related RNAs were found in human liver, baboon liver, and the human hepatoma cell line HepG2. None were detected in placenta, simian virus 40 (SV40)-transformed fibroblasts, and a lymphoblastoid cell line. The length of the mature apo B mRNA was estimated to be 18 kb, enough to code for a protein with a molecular weight in the neighborhood of 500,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=390482Documentos Relacionados
- Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B.
- Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B.
- Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b.
- Analysis of cDNA clones encoding the entire B-26 region of human apolipoprotein B.
- Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length.
