A pause site for RNA polymerase II is associated with termination of transcription.
AUTOR(ES)
Enriquez-Harris, P
RESUMO
Termination of transcription by RNA polymerase II has been postulated to involve a pausing process. We have identified such a pause signal, 350 bp into the 3' flanking region of the human alpha 2 globin gene at a position where termination is thought to occur. We show that this pause signal enhances the utilization of an upstream poly(A) site which is otherwise out-competed by a stronger downstream poly(A) site. We also demonstrate that the pause site rescues a poly(A) site that is inactive due to its location within an intron. Using nuclear run-on analysis we show that elongating RNA polymerase II molecules accumulate over this pause signal. Furthermore we show that when the pause site is positioned immediately downstream of a strong poly(A) signal, significant levels of transcription termination take place.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=452858Documentos Relacionados
- An RNA Polymerase Pause Site Is Associated with the Immunoglobulin μs Poly(A) Site
- In vitro analysis of a transcription termination site for RNA polymerase II.
- Transcriptional pause, arrest and termination sites for RNA polymerase II in mammalian N- and c-myc genes.
- CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription.
- RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor
