A positive screen for cloning PCR products.
AUTOR(ES)
Keese, P
RESUMO
We have developed a positive screen for cloning PCR products based on translational activation of lacZ. A vector with a translationally deficient lacZ alpha gene has been made by deletion of the Shine-Dalgarno sequence and initiation codon. The Shine-Dalgarno sequence and initiation codon are incorporated into one of the PCR primers to allow complementation by the PCR product of the inactive lacZ alpha gene, which results in blue transformed bacterial colonies. This screen allows more efficient detection of clones containing inserts made by PCR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146101Documentos Relacionados
- Coincidence cloning of Alu PCR products.
- Hetero-stagger cloning: efficient and rapid cloning of PCR products.
- Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
- Simplified method for confirmation of PCR products.
- A novel rapid method for detection of PCR products.
