A protein–protein interaction map of the Caenorhabditis elegans 26S proteasome
AUTOR(ES)
Davy, Anne
FONTE
Oxford University Press
RESUMO
The ubiquitin-proteasome proteolytic pathway is pivotal in most biological processes. Despite a great level of information available for the eukaryotic 26S proteasome—the protease responsible for the degradation of ubiquitylated proteins—several structural and functional questions remain unanswered. To gain more insight into the assembly and function of the metazoan 26S proteasome, a two-hybrid-based protein interaction map was generated using 30 Caenorhabditis elegans proteasome subunits. The results recapitulate interactions reported for other organisms and reveal new potential interactions both within the 19S regulatory complex and between the 19S and 20S subcomplexes. Moreover, novel potential proteasome interactors were identified, including an E3 ubiquitin ligase, transcription factors, chaperone proteins and other proteins not yet functionally annotated. By providing a wealth of novel biological hypotheses, this interaction map constitutes a framework for further analysis of the ubiquitin-proteasome pathway in a multicellular organism amenable to both classical genetics and functional genomics.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1084039Documentos Relacionados
- Protein–protein interaction map for yeast TFIID
- A protein–protein interaction map of yeast RNA polymerase III
- Interaction of the Doa4 Deubiquitinating Enzyme with the Yeast 26S Proteasome
- Negative regulation of male development in Caenorhabditis elegans by a protein–protein interaction between TRA-2A and FEM-3
- Regulation of Repair by the 26S Proteasome