A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.
AUTOR(ES)
Donson, J
RESUMO
We have isolated, from maize streak virus (MSV) preparations, a population of 'nested' DNA molecules. These molecules have ribonucleotides covalently linked to the DNA species' discrete 5' deoxyribonucleotide terminus. The major species has a DNA sequence of 80 nucleotides which is complementary to a region 5' of two hairpin structures on the MSV genome, almost exclusively in an intergenic region. These molecules have been used to prime the synthesis in vitro of a complementary strand to virion DNA, initiating this reaction at one site on the genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557819Documentos Relacionados
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