A rapid cytosine-specific modification of E. coli tRNA Leu 1 by semicarbazide-bisulfite, a probe for polynucleotide conformations.
AUTOR(ES)
Negishi, K
RESUMO
Cytosine residues in 32P-labeled E. coli tRNA Leu 1 were modified by treatment of the tRNA with the semicarbazide-bisulfite reagents [Hayatsu, H. (1976) Biochemistry 15, 2677-2682]. Analysis of the modification sites showed that only four cytidine residues, i.e. C35, C53, C85 and C86, reacted. They were identical with the cytidines of this tRNA accessible to methoxyamine [Chang, S. E. and Ish-Horowicz, D. (1974) J. Mol. Biol. 84, 375-388] and the accessibility was consistent with the conformational features recognized for tRNA in general. The rapidity and the simple nature of this modification demonstrate that the semicarbazide-bisulfite reaction is a useful tool in studying conformations of polynucleotides.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342566Documentos Relacionados
- Effect of sodium bisulfite modification on the arginine acceptance of E. coli tRNA Arg.
- 1H NMR of valine tRNA modified bases. Evidence for multiple conformations.
- Cytosine-specific chemical probing of DNA using bromide and monoperoxysulfate.
- The effect of specific structural modification on the biological activity of E. coli arginine tRNA.
- Evidence that 16S RNA from E. coli can assume two different biologically active conformations.