A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.
AUTOR(ES)
Seif, I
RESUMO
We have adapted a rapid sequencing technique from the enzymatic nick-translation method of Maat and Smith. The Forward-Backward procedure employs both synthetic and 3' to 5' exonucleolytic activities of E. coli DNA polymerase I to achieve greater reliability, especially in reading stretches of the same nucleotide. The technique has been employed to determine sequence alterations in four early SV40 temperature-sensitive (tsA) point mutants and five early SV40 viable deletion mutants. The nucleotide sequence of these mutants provides an insight into their biological properties.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324074Documentos Relacionados
- Isolation and characterization of simian virus 40 early region deletion mutants.
- DNA sequence alterations responsible for the synthesis of thermosensitive VP1 in temperature-sensitive BC mutants of simian virus 40.
- DNA sequences of polyoma virus early deletion mutants.
- Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
- Viable deletion mutants in the simian virus 40 early region.
