A rapid method for detecting specific amplified PCR fragments in microtiter plates.
AUTOR(ES)
Ortiz, A
RESUMO
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146076Documentos Relacionados
- A temperature regulator for microtiter plates.
- Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates.
- Mini-transfections on 96-well microtiter plates.
- Enzyme-linked immunosorbent assay for rubella immunoglobulin G: new method for attachment of antigens to microtiter plates.
- Titration of dengue viruses by immunofluorescence in microtiter plates.
