A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.
AUTOR(ES)
Woodland, R M
RESUMO
A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to immunofluorescence for detecting C psittaci inclusions. Its sensitivity for detection C trachomatis inclusions by dark field microscopy was similar to that of Giemsa staining.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=497741Documentos Relacionados
- Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.
- Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis.
- TestPack Chlamydia, a new rapid assay for the direct detection of Chlamydia trachomatis.
- Micro direct inoculation method for the isolation and identification of Chlamydia trachomatis.
- Evaluation of a novel solid-phase immunoassay, Clearview Chlamydia, for the rapid detection of Chlamydia trachomatis.
