A restriction endonuclease cleavage map of mouse mitochondrial DNA.
AUTOR(ES)
Moore, K H
RESUMO
A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonucleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI. The migration of linear DNA with a molecular weight greater than 1 X 10(6) was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343755Documentos Relacionados
- Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.
- A restriction endonuclease cleavage map of mitochondrial DNA from transformed hamster cells.
- Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA.
- Altered activity of restriction endonuclease Mn1-I cleavage of mouse satellite DNA.
- Identification of unintegrated forms of Kirsten murine sarcoma viral DNA and restriction endonuclease cleavage map of linear DNA.