A role for H-NS in the thermo-osmotic regulation of virulence gene expression in Shigella flexneri.
AUTOR(ES)
Porter, M E
RESUMO
The role of the hns gene (coding for the curved-DNA-binding protein H-NS) in the thermo-osmotic regulation of Shigella flexneri virulence gene transcription was investigated. Two structural genes, mxiC and icsB, which are transcribed divergently on the high-molecular-weight virulence plasmid, were found to be transcriptionally inhibited in cultures grown in a low-osmolarity medium, even at the inducing temperature. This repression was relieved by inactivation of the hns gene, establishing a role for hns in the osmotic as well as the thermal regulation of invasion gene expression. The physiological relevance of this finding is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=205622Documentos Relacionados
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