A Selective Medium for Quantitative Reisolation of Trichoderma harzianum from Agaricus bisporus Compost
AUTOR(ES)
Williams, Josie
FONTE
American Society for Microbiology
RESUMO
We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=165142Documentos Relacionados
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