A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
AUTOR(ES)
Anderson, S
RESUMO
In this paper we describe the synthesis and cloning of a short segment of DNA complementary to the region immediately adjacent to the EcoRI insertion site in the single-stranded bacteriophage vector M13mp2. This segment is useful as a "universal" primer for DNA sequencing by the dideoxynucleotide chain termination method; the template can be any DNA species cloned in M13mp2 or its derivatives. The primer has been cloned into the tetracycline resistance gene of plasmid pBR322 as one strand of a 26 bp EcoRI/BamHI fragment. This fragment may be readily prepared from an EcoRI + BamHI restriction digest of the parent plasmid (designated pSP14) by a simple size fractionation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324032Documentos Relacionados
- Mutations induced by methylene blue plus light in single-stranded M13mp2.
- Single-stranded M13 DNA: use as a cloning vector.
- Specificity of ionizing radiation-induced mutagenesis in the lac region of single-stranded phage M13 mp10 DNA.
- One step DNA sequencing of single-stranded DNA with reverse dye-primer.
- Conformations of the Single-Stranded DNA of Bacteriophage M13*
