A Simple Autoradiographic Technique for Studying Diffusion of Water into Seeds 1

AUTOR(ES)
RESUMO

Visual observations on rates and modes of water penetration into black bean seeds and into wheat and sorghum kernels during conditioning were accomplished by an autoradiographic procedure that eliminates a freezing microtome and liquid and stripping film emulsions. Seeds soaked in tritiated H2O were hand sectioned before freeze-quenching in liquid N2 and subsequent block autoradiography on nuclear medicine film.

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