A Simple Technique for the Establishment of Nitrogenase in Soybean Callus Culture 12
AUTOR(ES)
Child, J. J.
RESUMO
A nitrogen-fixing association can be readily established in vitro between Rhizobium and cultured soybean root cells. Plant cells are grown as a thin callus on the surface of solid defined medium containing low levels of inorganic nitrogen and inoculated with bacteria during the active phase of growth. Acetylene reduction activities up to 275 nmoles ethylene/hr·g dry weight of cells have been recorded.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541339Documentos Relacionados
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