A structure of potentially active and inactive genes of chicken erythrocyte chromatin upon decondensation.
AUTOR(ES)
Kukushkin, A N
RESUMO
In the presence of 3 mM MgCl2 DNase I cleavage of bulk, globin and ovalbumin gene chromatin in chicken erythrocyte nuclei generates fragments which are multiples of a double-nucleosome repeat. However, in addition to the dinucleosomal periodicity beta-globin gene chromatin was fragmented into multiples of a 100 b.p. interval which is characteristic for partially unfolded chromatin. This distinction correlates with higher sensitivity of beta-globin domain to DNase I and DNase II as compared to the inactive ovalbumin gene. At 0.7 mM MgCl2 where these DNases fragment bulk chromatin into series of fragments with a 100 b.p. interval, the difference in digestibility of the investigated genes is dramatically decreased. When chromatin has been decondensed by incubation of nuclei in 10 mM Tris-buffer, DNase II generates a typical nucleosomal repeat, and the differential nuclease sensitivity of the analyzed genes is not observed. The data suggest that higher nuclease sensitivity of potentially active genes is due to irregularities in higher order chromatin structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338576Documentos Relacionados
- Chromatin structure of active and inactive human X chromosomes.
- Chromatin structure of transcriptionally active and inactive human c-myc alleles.
- Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.
- Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.
- Loosened nucleosome linker folding in transcriptionally active chromatin of chicken embryo erythrocyte nuclei.
