A Study of the Aminoacyl-sRNA Synthetases of Phaseolus vulgaris in Relation to Germination
AUTOR(ES)
Anderson, J. W.
RESUMO
Optimum conditions for the extraction and assay by ATP-pyrophosphate exchange of the aminoacyl-sRNA synthetases of the various tissues of french bean (Phaseolus vulgaris) seeds and seedlings are described. Extracts of plumules, after passage through Sephadex G-25, were assayed for synthetase activity using an amino acid mixture as substrate, when a 30 to 100-fold stimulation of exchange above the endogenous level was obtained. This marked enhancement of exchange by added amino acids is largely attributed to the use of dilute extracts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=396038Documentos Relacionados
- Aminoacyl-sRNA synthetases as possible regulators of protein synthesis in the embryo of the sea urchin Paracentrotus lividus.
- NaF inhibition of the initial binding of aminoacyl-sRNA to reticulocyte ribosomes.
- EVIDENCE FOR DEGENERACY AND AMBIGUITY IN INTERSPECIES AMINOACYL-sRNA FORMATION
- THE EFFECT OF RIBONUCLEASE DIGESTS OF AMINOACYL-sRNA ON A PROTEIN SYNTHESIS SYSTEM*
- Interspecies aminoacyl-sRNA formation: fractionation of Neurospora enzymes involved in anomalous aminoacylation.