A suppressor of mutations in the region adjacent to iterons of pSC101 ori.
AUTOR(ES)
Ohkubo, S
RESUMO
Some single-base changes in a 14-bp region (the downstream region) adjacent to three repeated sequences (iterons) in pSC101 ori are very deleterious for replication. We isolated a host suppressor mutation for one of these mutations and found that the suppressor suppressed all the mutations tested in the downstream region. The nucleotide sequence of the suppressor revealed that the suppressor gene was identical to dksA, which encodes a multicopy suppressor of the heat shock gene dnaK.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178940Documentos Relacionados
- Minimal essential origin of plasmid pSC101 replication: requirement of a region downstream of iterons.
- Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: mutations in the topA gene allow pSC101 replication in the absence of IHF.
- Boundaries of the pSC101 minimal replicon are conditional.
- Revised Interpretation of the Origin of the pSC101 Plasmid
- Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: characterization of pSC101 mutants that replicate in the absence of IHF.
